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您的位置:首頁>>生命科學>>抗原/抗體>>345013 PE/Cyanine7 anti-human CD366 (Tim-3) Antibody
Cat. Number
345013
Chemical Name
345013 PE/Cyanine7 anti-human CD366 (Tim-3) Antibody
Qty 1
100tests
Storage condition
2°C and 8°C
References
  • Isotype Control:PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • Verified Reactivity:Human

  • Antibody Type:Monoclonal

  • Host Species:Mouse

  • Immunogen:Human Tim-3 fusion protein

  • Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

  • Preparation:The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

  • Concentration:Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

  • Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

  • Application:FC - Quality tested

  • Recommended Usage:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μl per million cells in 100 μl staining volume or 5 μl per 100 μl of whole blood.

  • Excitation Laser:Blue Laser (488 nm)

  • Green Laser (532 nm)/Yellow-Green Laser (561 nm)

  • Application Notes:Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.

  • Additional Product Notes:BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

  • Application References:(PubMed link indicates BioLegend citation)

    1. Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)

    2. Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)

    3. Klibi J, et al 2009. Blood 113:1957. (FC, Block)

  • Product Citations

    1. Jespersen H, et al. 2017. Nat Commun. 10.1038/s41467-017-00786-z. PubMed

    2. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed

    3. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed

    4. Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed

    5. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed

    6. Fisher J, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.03.002. PubMed

    7. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed

    8. Riberdy JM, et al. 2020. Mol Ther Methods Clin Dev. 1.146527778. PubMed

    9. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed

  • RRID

  • AB_2561719 (BioLegend Cat. No. 345013)
    AB_2561720 (BioLegend Cat. No. 345014)


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