<span id="v9r9v"><video id="v9r9v"></video></span>
<strike id="v9r9v"><video id="v9r9v"></video></strike>
<th id="v9r9v"></th>
<th id="v9r9v"></th>
<span id="v9r9v"><video id="v9r9v"></video></span>
<progress id="v9r9v"><noframes id="v9r9v"><span id="v9r9v"><noframes id="v9r9v">
您的位置:首頁>>生命科學>>抗原/抗體>>349914 PE/Cyanine7 anti-human CD152 (CTLA-4) Antibody
Cat. Number
349914
Chemical Name
349914 PE/Cyanine7 anti-human CD152 (CTLA-4) Antibody
Qty 1
100tests
Storage condition
2°C and 8°C
References
  • Isotype Control:PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • Verified Reactivity:Human

  • Antibody Type:Monoclonal

  • Host Species:Mouse

  • Immunogen:Extracellular domain of human CTLA-4 and human IgG1 Fc fusion protein

  • Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

  • Preparation:The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

  • Concentration:Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

  • Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

  • Application:ICFC - Quality tested

  • Recommended Usage:Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per million cells in 100 μL staining volume or 5 μL per 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

  • Excitation Laser:Blue Laser (488 nm)

  • Green Laser (532 nm)/Yellow-Green Laser (561 nm)

  • Application Notes:ELISA Detection: The biotinylated L3D10 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified A3.6B10.G1 antibody (Cat. No. 525401) as the capture antibody and recombinant human CTLA-4 (Cat. No. 591909) as the standard.


  • Flow Cytometry: The fluorochrome-labeled L3D10 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify CTLA-4-producing cells within mixed cell populations.

    Note: For testing human soluble CTLA-4 in serum, plasma or cell culture supernatant, LEGEND MAX? Human Soluble CTLA-4 ELISA Kit with Pre-coated Plates (Cat. No. 437407) are specially developed and recommended.

    Additional reported applications (for the relevant formats) include: Blocking of CTLA-4/B7-1 interaction and blocking of CTLA-4-mediated inhibitory function to promote T cell expansion1,2.

    This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of frozen or paraffin-embedded tissue sections (IHC-F or IHC-P).

  • Additional Product Notes:BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

  • Application References:
    (PubMed link indicates BioLegend citation)

    1. May K, et al. 2005. Blood 105:1114. (Block)

    2. Lute K, et al. 2005. Blood 106:3127. (Block)

  • Product Citations

    1. Sander F, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-2040-9. PubMed

    2. Zheng Z, et al. 2017. PLoS One. 10.1371/journal.pone.0184127. PubMed

    3. Li X, et al. 2019. Front Oncol. 1.115277778. PubMed

    4. Wang A, et al. 2020. Front Immunol. 1.213194444. PubMed

    5. RY H, et al. 2016. Oncoimmunology. 6:e1249561. PubMed

    6. Luoma AM, et al. 2020. Cell. 182(3):655-671.e22. PubMed

    7. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed

  • RRID

  • AB_2563097 (BioLegend Cat. No. 349913)
    AB_2563098 (BioLegend Cat. No. 349914)


在線咨詢 聯系方式 二維碼

服務熱線

021-60498804

掃一掃,關注我們

人妻a∨中文字幕,亚洲AV日韩AV永久无码绿巨人,一国产永久视频,2022一本久道久久综合狂躁,国产欧美日韩在线视频一区 日韩精品Aⅴ无码AV 狠狠久夜一区二区三区 亚洲国产精品无码高潮 精品无码一区二区三在线观看 專業选择!国际品质好吊操视频 中文字幕一区二区人妻本田岬 久久综合电影一区 日韩AV不卡一区二区三区无码 欧美色欧美亚洲日韩在线播放