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您的位置:首頁>>生命科學>>抗原/抗體>>E-006 新型冠狀病毒(COVID-19)RBD蛋白 (2019-nCoV)Spike Protein(RBD,mFc Tag)
Cat. Number
E-006
Chemical Name
E-006 新型冠狀病毒(COVID-19)RBD蛋白 (2019-nCoV)Spike Protein(RBD,mFc Tag)
CAS Number
E006
Qty 1
1mg
Qty 2
100mg
Application Notes
> 95 % as determined by SDS-PAGE.
Synonym
RBD蛋白mFc Tag;cov spike Protein, 2019-nCoV;coronavirus spike Protein, 2019-nCoV
Storage condition
-20℃ to -80℃
References

2019-nCoV Spike Protein (RBD, Fc Tag)
新型冠狀病毒RBD蛋白產品信息

純度:> 95 % as determined by SDS-PAGE.

蛋白質序列:RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFADDDDKAVPRDSGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK

內毒素< 1.0 EU per μg protein as determined by the LAL method.

生物活性

    Measured by its binding ability in a functional ELISA.
Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (RBD, mFc Tag),the EC50 of 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50ng/mL.

蛋白構建

    A DNA sequence encoding the NCP-CoV(2019-nCoV) Spike Protein (RBD) was expressed with the Fc region of mouse IgG1 at the C-terminus.

表達宿主:HEK293 Cells

種屬:2019-nCoV

預測 N 端:Arg

分子量The recombinant NCP-CoV(2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.

緩沖液    Lyophilized from sterile PBS, pH 7.4.

Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

運輸方式

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

穩定性 & 儲存條件

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

復溶

    A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

研究領域

  • Microbiology>>Pathogenic microorganism>>viruses>>Animal virus>>RNA virus>>+ssRNA virus>>Coronaviridae>>Coronavirus (CoV)>>CoV viral antigen

參考文獻

  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.

  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.

  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

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上?;菡\生物GMP級細胞培養產品列表:

貨號名稱規格
GMP-001重組抗PD-1全人單克隆抗體1.0mg
GMP-002重組Her-2全人單克隆抗體2mg/10mg
GMP-003鼠抗人CD3單克隆抗體0.5mg/1mg
GMP-004鼠抗人CD28單克隆抗體0.5mg/1mg
GMP-005重組人Activin A50ug/1.0mg
GMP-006重組人腦源性神經營養因子(rhBDNF)50ug/1.0mg
GMP-007重組人堿性成纖維細胞生長因子(rhbFGF)50ug/1.0mg
GMP-008重組人BMP-2(rhBMP-2)50ug/1.0mg
GMP-009重組人BMP-4(rhBMP-4)50ug/1.0mg
GMP-010重組人BMP-7(rhBMP-7)50ug/1.0mg
GMP-011重組人CD40L(rhCD40L)50ug/1.0mg
GMP-012重組人Dkk1(rhDkk1)50ug/1.0mg
GMP-013重組人表皮生長因子(rhEGF)1.0mg
GMP-014重組人促紅細胞生成素(rhEPO)1.0mg
GMP-015重組人Flt3L(rhFLt3L)50ug/1.0mg
GMP-016重組人角質細胞生長因子-2(rhKGF-2&FGF-10)50ug/1.0mg
GMP-017重組人成纖維細胞生長因子-4(rhFGF4)50ug/1.0mg
GMP-018重組人成纖維細胞生長因子-9(rhFGF9)50ug/1.0mg
GMP-019重組人纖維連接蛋白(rhFN)1mg
GMP-020重組人膠質細胞源性神經營養因子(rhGDNF)50ug/1.0mg
GMP-021重組人肝細胞生長因子(rhHGF)50ug/1.0mg
GMP-022重組人干擾素-γ(rhIFN-γ)100ug/1.0mg
GMP-023重組人白細胞介素-1a(rhIL-1a)50ug/1.0mg
GMP-024重組人白細胞介素-2(rhIL-2)100ug/1.0mg
GMP-025重組人白細胞介素-3(rhIL-3)50ug/1.0mg
GMP-026重組人白細胞介素-4(rhIL-4)50ug/1.0mg
GMP-027重組人白細胞介素-6(rhIL-6)50ug/1.0mg
GMP-028重組人白細胞介素-7(rhIL-7)50ug/1.0mg
GMP-029重組人白細胞介素-12(rhIL-12)50ug/1.0mg
GMP-030重組人白細胞介素-15(rhIL-15)50ug/1.0mg
GMP-031重組人白細胞介素-18(rhIL-18)50ug/1.0mg
GMP-032重組人白細胞介素-21(rhIL-21)50ug/1.0mg
GMP-033重組人白血病抑制因子(rhLIF)50ug/1.0mg
GMP-034重組人長鏈胰島素樣生長因子-1(rhLR3-IGF-1)1.0mg
GMP-035重組人Noggin(rhNoggin)50ug/1.0mg
GMP-036重組人NRG-1(rhNRG-1)50ug/1.0mg
GMP-037重組人血小板源生長因子(rhPDGF-BB)1.0mg
GMP-038重組人R-spondin(rhR-spondin)50ug/1.0mg
GMP-039重組人干細胞因子(rhSCF)50ug/1.0mg
GMP-040重組人音猬因子(rhSHH)50ug/1.0mg
GMP-041重組人腫瘤壞死因子(rhTNF-a)50ug/1.0mg
GMP-042重組人轉化生長因子(rhTGFβ1)50ug/1.0mg
GMP-043重組人血管內皮生長因子(rhVEGF)50ug/1.0mg
GMP-044重組人Wnt-3α(rhWnt-3α)50ug/1.0mg
GMP-045重組人胰島素(rhInsulin)1.0g/10.0g
GMP-046牛轉鐵蛋白(Bovine Transferrin) inactivated virus1.0g/10.0g
GMP-047重組人干擾素α2b1.0mg
GMP-048重組人粒細胞集落刺激因子1.0mg
E-001重組CRISPR-Cas9蛋白100/500pmol
E-002重組CRISPR-Cas12a蛋白100/2000pmol
E-003重組CRISPR-Cas13a蛋白100/500pmol
E-004重組SUMO蛋白酶1000U
E-005重組TEV蛋白酶1000/10000U
E-006Spike Protein (RBD, mouseFc Tag)1mg/10mg
E-007Spike Protein (S1 Subunit, His Tag)1mg/10mg
VISC2-S002重組全長S蛋白 100ug/1.0mg
VISC2-RB04RBD蛋白 (RBD Subunit,His Tag)100ug/1.0mg
VISC2-RB05RBD-591蛋白(RBD-591 Subunit,His Tag)100ug/1.0mg

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